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| Registros recuperados : 10 | |
| 1. |  | AREVALO, A.P.; BASIKA, T.; ANCHETA, S.; PERELMUTER, K.; RICCIARDI, A.; BOLLATI-FOGOLÍN, M.; CRISPO, M. 3R's applied to in vivo biological activity of recombinant human erythropoietin assay. Technical Article. Biological Models Research and Technology Journal. 2023, Volume 3, Issue 1, e00012023. http://dx.doi.org/10.4322/2675-9225.00012023 -- OPEN ACCESS. Article history: Submitted date 9 February 2023, Accepted date 19 April 2023. -- Correspondence: Crispo, M.; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; email:crispo@pasteur.edu.uy --...| Biblioteca(s): INIA Las Brujas. |
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| 2. | | KALDS, P.; CRISPO, M.; TESSON, L.; ANEGÓN, I.; CHEN KEY, Y.; WANG, X.; MENCHACA, A. Generation of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene. Chapter 16. Methods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16| Biblioteca(s): INIA La Estanzuela. |
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| 3. | | DOS SANTOS-NETO, P.C.; CUADRO, F.; SOUZA-NEVES, M.; CRISPO, M.; MENCHACA, A. Refinements in embryo manipulation applied to CRISPR technology in livestock. Theriogenology. 2023, Volume 208, Pages 142-148. https://doi.org/10.1016/j.theriogenology.2023.05.028 Article history: Received 14 April 2023; Received in revised form 29 May 2023; Accepted 29 May 2023; Available online 9 June 2023. -- Correspondence author: Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy,...| Biblioteca(s): INIA Las Brujas. |
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| 4. | | CIUFFO, C.; ARÉVALO, A.P.; GARCIA, F.; PERDOMO, T.; CRISPO, M.; GIANNITTI, F.; BUSCHIAZZO, A.; ZARANTONELLI, L. Estudio y caracterización de una variante autóctona de Leptospira para su potencial uso como antígeno en vacunas de uso veterinario. In: Jornadas Uruguayas de Buiatría, 49., 2022. Paysandú, Uruguay: Centro Médico Veterinario de Paysandú; Filial de la Sociedad de Medicina Veterinaria del Uruguaya; Sociedad Uruguaya de Buiatría, 9 al 11 Junio 2022. p.192-194. p.192-194.| Biblioteca(s): INIA Las Brujas. |
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| 5. | | CIUFFO, C.; ARÉVALO, A.P.; GARCIA, F.; PERDOMO, T.; CRISPO, M.; GIANNITTI, F.; BUSCHIAZZO, A.; ZARANTONELLI, L. Estudio de la capacidad infectiva, colonización renal e inmunogenicidad de Leptospira interrogans serogrupo Pomona serovar Kennewicki en un modelo murino sub-letal y crónico. [abstract]. Sub-área 4: Patogenia, virulencia y resistencia. In: Anais III Consensos Latinoamericanos em Leptospirose Animal. Nov 30-Dic 02, 2021 (evento online).| Biblioteca(s): INIA Las Brujas. |
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| 6. | | CRISPO, M.; CHENOUARD, V.; DOS SANTOS-NETO P; TESSON, L.; SOUZA-NEVES, M.; HESLAN, J.M.; CUADRO, F.; ANEGÓN, I.; MENCHACA, A. Generation of a Human Deafness Sheep Model Using the CRISPR/Cas System. Chapter 12. Methods in Molecular Biology, 2022, Volume 2495, Pages 233-258. doi: https://doi.org/10.1007/978-1-0716-2301-5_12| Biblioteca(s): INIA La Estanzuela. |
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| 7. | | ALTIER, N.; ABREO, E.; BEYHAUT, E.; GARAYCOCHEA, S.; TORRES, P.; CERECETTO, V.; MARTÍN, N.; CUITIÑO, M.J.; CRISPO, M.; ARÉVALO, A.P.; REGO, N.; ARROSPIDE, G.; LAGE, M.; SUNDBERG, G. Desarrollo de un biofertilizante microbiano para aumentar la disponibilidad de fósforo en el cultivo de soja. * Sustentabilidad. Revista INIA Uruguay, 2020, no.62, p.95-100. (Revista INIA; 62) *Proyecto: Desarrollo de inoculantes para la movilización de fósforo como insumo en la producción agrícola. INIA/IP/EMPRESAS. Proyecto financiado
por la herramienta Alianzas para la Innovación de la Agencia Nacional de Investigación e...| Biblioteca(s): INIA Las Brujas. |
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| 8. | | CABRERA, A.; FRESIA, P.; BERNÁ, L.; SILVEIRA, C.S.; MACÍAS-RIOSECO, M.; AREVALO, A.P.; CRISPO, M.; PRITSCH, O.; RIET-CORREA, F.; GIANNITTI, F.; GIANNITTI, F.; FRANCIA, M.E.; ROBELLO, C. Isolation and molecular characterization of four novel Neospora caninum strains. Genetics, Evolution, and Phylogeny - Short Communication. Parasitology Research, 1 December 2019, Volume 118, Issue 12, Pages 3535-3542. Doi: 10.1007/s00436-019-06474-9 Article history: Received: 11 April 2019 / Accepted: 24 September 2019 / Published online: 7 November 2019.
Funding Sponsor: Agencia Nacional de Investigación e Innovación (ANII).
Funding Text: This project was funded by grant...| Biblioteca(s): INIA Las Brujas. |
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| 9. | | PASSOS, J. R. S.; GUERREIRO, D. D.; OTÁVIO, K. S.; SANTOS-NETO, P. C. DOS; SOUZA-NEVES, M.; CUADRO, F.; NUÑEZ-OLIVERA, R.; CRISPO, M.; BEZERRA, M. J. B.; SILVA, R. F.; LIMA, L. F.; FIGUEIREDO, J. R.; BUSTAMANTE-FILHO, I. C.; MENCHACA, A.; MOURA, A. A. Global proteomic analysis of preimplantational ovine embryos produced in vitro. Reproduction in Domestic Animals, 2022, Volume 57, Issue 7; pages 784-797. doi: https://doi.org/10.1111/rda.14122 Article history: Received 15 February 2022; Accepted 1 April 2022. -- Funding text - The experiments presently described were conducted at the facilities of the (Fundacion IRAUy, Montevideo, Uruguay) and at the (UBAL) of the , Uruguay....| Biblioteca(s): INIA Las Brujas. |
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| 10. | | PASSOS, J.R.S.; GUERREIRO, D.D.; KAMILA S. OTÁVIO; DOS SANTOS-NETO, P.C.; SOUZA-NEVES, M.; CUADRO, F.; NUÑEZ?OLIVERA, R.; CRISPO, M.; VASCONCELOS, F.R.; BEZERRA, M.J.B.; SILVA, R.F.; LIMA, L.F.; FIGUEIREDO, J.R.; BUSTAMANTE-FILHO, I.C.; MENCHACA, A.; MOURA, A.A. How in vitro maturation changes the proteome of ovine cumulus-oocyte complexes?. Volume 89, Issue 10, Pages 459 - 470October 2022 Molecular reproduction and development, October 2022, Volume 89, Issue 10, pages 459-470. doi: https://doi.org/10.1002/mrd.23638 Article history: Received: 16 February 2022 | Accepted: 21 July 2022. -- Corresponding author: Moura, A.A.; Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil;...| Biblioteca(s): INIA La Estanzuela. |
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| Registros recuperados : 10 | |
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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
20/07/2022 |
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Actualizado : |
20/07/2022 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
CRISPO, M.; CHENOUARD, V.; DOS SANTOS-NETO P; TESSON, L.; SOUZA-NEVES, M.; HESLAN, J.M.; CUADRO, F.; ANEGÓN, I.; MENCHACA, A. |
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Afiliación : |
MARTINA CRISPO, Laboratory Animal Biotechnology Unit, Institut Pasteur de Montevideo, Montevideo, Uruguay.; VANESSA CHENOUARD, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; PEDRO DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; LAURENT TESSON, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; JEAN-MARIE HESLAN, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; FEDERICO CUADRO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; IGNACIO ANEGÓN, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay. |
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Título : |
Generation of a Human Deafness Sheep Model Using the CRISPR/Cas System. |
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Complemento del título : |
Chapter 12. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Methods in Molecular Biology, 2022, Volume 2495, Pages 233-258. doi: https://doi.org/10.1007/978-1-0716-2301-5_12 |
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DOI : |
10.1007/978-1-0716-2301-5_12 |
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Idioma : |
Inglés |
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Contenido : |
Abstract:
CRISPR/Cas9 system is a promising method for the generation of human disease models by genome editing in non-conventional experimental animals. Medium/large-sized animals like sheep have several advantages to study human diseases and medicine. Here, we present a protocol that describes the generation of an otoferlin edited sheep model via CRISPR-assisted single-stranded oligodinucleotide-mediated Homology-Directed Repair (HDR), through direct cytoplasmic microinjection in in vitro produced zygotes.Otoferlin is a protein expressed in the cochlear inner hair cells, with different mutations at the OTOF gene being the major cause of nonsyndromic recessive auditory neuropathy spectrum disorder in humans. By using this protocol, we reported for the first time an OTOF KI model in sheep with 17.8% edited lambs showing indel mutations, and 61.5% of them bearing knock-in mutations by HDR . The reported method establishes the bases to produce a deafness model to test novel therapies in human disorders related to OTOF mutations. |
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Palabras claves : |
Embryo; Genome editing; In vitro fertilization; OTOF; SsODNs. |
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Thesagro : |
OVEJA. |
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Asunto categoría : |
-- |
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Marc : |
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245 $aGeneration of a Human Deafness Sheep Model Using the CRISPR/Cas System.$h[electronic resource]
260 $c2022
520 $aAbstract: CRISPR/Cas9 system is a promising method for the generation of human disease models by genome editing in non-conventional experimental animals. Medium/large-sized animals like sheep have several advantages to study human diseases and medicine. Here, we present a protocol that describes the generation of an otoferlin edited sheep model via CRISPR-assisted single-stranded oligodinucleotide-mediated Homology-Directed Repair (HDR), through direct cytoplasmic microinjection in in vitro produced zygotes.Otoferlin is a protein expressed in the cochlear inner hair cells, with different mutations at the OTOF gene being the major cause of nonsyndromic recessive auditory neuropathy spectrum disorder in humans. By using this protocol, we reported for the first time an OTOF KI model in sheep with 17.8% edited lambs showing indel mutations, and 61.5% of them bearing knock-in mutations by HDR . The reported method establishes the bases to produce a deafness model to test novel therapies in human disorders related to OTOF mutations.
650 $aOVEJA
653 $aEmbryo
653 $aGenome editing
653 $aIn vitro fertilization
653 $aOTOF
653 $aSsODNs
700 1 $aCHENOUARD, V.
700 1 $aDOS SANTOS-NETO P
700 1 $aTESSON, L.
700 1 $aSOUZA-NEVES, M.
700 1 $aHESLAN, J.M.
700 1 $aCUADRO, F.
700 1 $aANEGÓN, I.
700 1 $aMENCHACA, A.
773 $tMethods in Molecular Biology, 2022, Volume 2495, Pages 233-258. doi: https://doi.org/10.1007/978-1-0716-2301-5_12
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